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SEPHADEX G-50

Description Uses
SEPHADEX G-50
SEPHADEX G-50 structure
CAS No.
9048-71-9
Chemical Name:
SEPHADEX G-50
Synonyms
PDXTM G.F.;SEPHADEX G-50;SEPHADEX(R) G-50;Sephadex G50, BR;SEPHADEX G-50 FINE;PDX GF 50 (50-150);PDX GF 50 (100-300);SEPHADEX G-50 COARSE;Sephadex G-50 60-100;SEPHADEX G-50 MEDIUM
CBNumber:
CB3728066
Molecular Formula:
NULL
Formula Weight:
0
MOL File:
Mol file

SEPHADEX G-50 Properties

CAS DataBase Reference
9048-71-9
SAFETY
  • Risk and Safety Statements
WGK Germany  3
HS Code  39140000

SEPHADEX G-50 price More Price(4)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich G5080 Sephadex? G-50 Fine 9048-71-9 50g $356 2020-12-02 Buy
Sigma-Aldrich G5080 Sephadex? G-50 Fine 9048-71-9 100g $525 2020-12-02 Buy
Sigma-Aldrich G5080 Sephadex? G-50 Fine 9048-71-9 500g $1910 2020-12-02 Buy
Sigma-Aldrich G5080 Sephadex? G-50 Fine 9048-71-9 10g $99.3 2020-12-02 Buy

SEPHADEX G-50 Chemical Properties,Uses,Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for purification of DNA from small molecules by gel filtration
  3. Small bead size for shorter diffusion distances
  4. Smallest bead size in the Sephadex G-50 range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.
 
lower limit
upper limit
dextran gels
Sephadex G-50
1.5
30
Sephadex G-75
3
80
Sephadex G-100
4
150
Sephadex G-150
5
300
Sephadex G-200
5
600
polyacrylamide gels
Bio-Gel P-10
1.5
20
Bio-Gel P-30
2.5
40
Bio-Gel P-60
3
60
Bio-Gel P-100
5
100
Bio-Gel P-150
15
150
BioGel P-200
30
200
Bio-Gel P-300
60
400
dextran-polyacrylamide gels
Sephacryl S-200
5
250
Sephacryl S-300
10
1500
Sephacryl S-400
20
8000
agarose gels
Sepharose 6B
10
4000
Sepharose 4B
60
20,000
Sepharose 2B
70
40,000
Bio-Gel A-0.5
10
500
Bio-Gel A-1.5
10
1500
Bio-Gel A-5
10
5000
Bio-Gel A-15
40
15,000
Bio-Gel A-50
100
50,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products


SEPHADEX G-50 Suppliers

Global( 104)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3623 58
+86-371-86658258
sales@coreychem.com CHINA 30043 58
BOC Sciences
1-631-619-7922
1-631-614-7828 inquiry@bocsci.com United States 20039 58
+86-13650506873
sales@chemdad.com CHINA 35434 58
400-0066-400
021-55660885 sales@jonln.com;sales@jonln.com China 2000 65
025-83697070
+86-25-83453306 info@chemlin.com.cn China 19991 64
18981987031 400-1166-196
cdhxsj@163.com;1955352637@qq.com;cdhxsj@163.com China 9897 57
400 638 7771
sales@heowns.com China 14460 57
86-21-63210123
86-21-63290778 86-21-63218885 sj_scrc@sinopharm.com China 9848 79
13308453923 0731-85529965-
0731-85526065 3002708854@qq.com;3002778010@qq.com;3002732518@qq.com China 3362 62

View Lastest Price from SEPHADEX G-50 manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2019-12-25 Sephadex G-50
9048-71-9
US $1.00 / kg 1kg 95%-99% 1000kg career henan chemical co

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